看看这个 MSDN Forum Posting ，它建议将单元格值与 Cell.TrueValue 进行比较。

所以按照它的例子，你的代码应该是这样的：(这是完全未经测试的)

编辑:似乎未绑定DataGridViewCheckBox的Cell.TrueValue的默认值是null，您需要在列定义中设置它。

private void chkItems_CheckedChanged(object sender, EventArgs e)
    foreach (DataGridViewRow row in dataGridView1.Rows)
        DataGridViewCheckBoxCell chk = (DataGridViewCheckBoxCell)row.Cells[1];
        if (chk.Value  == chk.TrueValue)
            chk.Value = chk.FalseValue;
            chk.Value = chk.TrueValue;
}

下面的代码是在构造函数中设置TrueValue和FalseValue以及检查是否为空的工作说明：

public partial class Form1 : Form
    public Form1()
        InitializeComponent();
        DataGridViewCheckBoxColumn CheckboxColumn = new DataGridViewCheckBoxColumn();
        CheckboxColumn.TrueValue = true;
        CheckboxColumn.FalseValue = false;
        CheckboxColumn.Width = 100;
        dataGridView1.Columns.Add(CheckboxColumn);
        dataGridView1.Rows.Add(4);
    private void chkItems_CheckedChanged(object sender, EventArgs e)
        foreach (DataGridViewRow row in dataGridView1.Rows)
            DataGridViewCheckBoxCell chk = (DataGridViewCheckBoxCell)row.Cells[0];
            if (chk.Value == chk.FalseValue || chk.Value == null)
                chk.Value = chk.TrueValue;
                chk.Value = chk.FalseValue;
        dataGridView1.EndEdit();
}

当我看到这篇文章没有得到回复时，我正在制作我自己版本的复选框来控制一个DataGridViewCheckBoxColumn。要设置DataGridViewCheckBoxCell的选中状态，请执行以下操作：

foreach (DataGridViewRow row in dataGridView1.Rows)
    dataGridView1.Rows[row.Index].SetValues(true);
}

对于任何其他想要完成同样的事情的人来说，这是我想出来的。

这使得这两个控件的行为类似于Gmail中的复选框列。它保留了鼠标和键盘的功能。

using System;
using System.Windows.Forms;
namespace Check_UnCheck_All
    public partial class Check_UnCheck_All : Form
        public Check_UnCheck_All()
            InitializeComponent();
            dataGridView1.RowCount = 10;
            dataGridView1.AllowUserToAddRows = false;
            this.dataGridView1.CellContentClick += new System.Windows.Forms.DataGridViewCellEventHandler(this.dgvApps_CellContentClick);
            this.dataGridView1.CellMouseUp += new System.Windows.Forms.DataGridViewCellMouseEventHandler(this.myDataGrid_OnCellMouseUp);
            this.dataGridView1.CellValueChanged += new System.Windows.Forms.DataGridViewCellEventHandler(this.myDataGrid_OnCellValueChanged);
            this.checkBox1.Click += new System.EventHandler(this.checkBox1_Click);
        public int chkInt = 0;
        public bool chked = false;
        public void myDataGrid_OnCellValueChanged(object sender, DataGridViewCellEventArgs e)
            if (e.ColumnIndex == dataGridView1.Rows[0].Index && e.RowIndex != -1)
                DataGridViewCheckBoxCell chk = dataGridView1.Rows[e.RowIndex].Cells[0] as DataGridViewCheckBoxCell;
                if (Convert.ToBoolean(chk.Value) == true) chkInt++;
                if (Convert.ToBoolean(chk.Value) == false) chkInt--;
                if (chkInt < dataGridView1.Rows.Count && chkInt > 0)
                    checkBox1.CheckState = CheckState.Indeterminate;
                    chked = true;
                else if (chkInt == 0)
                    checkBox1.CheckState = CheckState.Unchecked;
                    chked = false;
                else if (chkInt == dataGridView1.Rows.Count)
                    checkBox1.CheckState = CheckState.Checked;
                    chked = true;
        public void myDataGrid_OnCellMouseUp(object sender, DataGridViewCellMouseEventArgs e)
            // End of edition on each click on column of checkbox
            if (e.ColumnIndex == dataGridView1.Rows[0].Index && e.RowIndex != -1)
                dataGridView1.EndEdit();
            dataGridView1.BeginEdit(true);
        public void dgvApps_CellContentClick(object sender, DataGridViewCellEventArgs e)
            if (dataGridView1.CurrentCell.GetType() == typeof(DataGridViewCheckBoxCell))
                if (dataGridView1.CurrentCell.IsInEditMode)
                    if (dataGridView1.IsCurrentCellDirty)
                        dataGridView1.EndEdit();
                dataGridView1.BeginEdit(true);
        public void checkBox1_Click(object sender, EventArgs e)
            if (chked == true)
                foreach (DataGridViewRow row in dataGridView1.Rows)
                    DataGridViewCheckBoxCell chk = (DataGridViewCheckBoxCell)row.Cells[0];
                    if (chk.Value == chk.TrueValue)
                        chk.Value = chk.FalseValue;
                        chk.Value = chk.TrueValue;
                chked = false;
                chkInt = 0;
                return;
            if (chked == false)
                foreach (DataGridViewRow row in dataGridView1.Rows)
                    dataGridView1.Rows[row.Index].SetValues(true);
                chked = true;
                chkInt = dataGridView1.Rows.Count;
}

对你来说很简单的代码它会工作的

private void dgv_CellClick(object sender, DataGridViewCellEventArgs e)
    if (dgv.CurrentRow.Cells["ColumnNumber"].Value != null && (bool)dgv.CurrentRow.Cells["ColumnNumber"].Value)
        dgv.CurrentRow.Cells["ColumnNumber"].Value = false;
        dgv.CurrentRow.Cells["ColumnNumber"].Value = null;