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(2) Robbins T; Liu Y-C; Cane DE; Khosla C Structure and Mechanism of Assembly Line Polyketide Synthases . Curr. Opin. Struct. Biol 2016, 41 , 10–18. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(3) Lowry B; Li X; Robbins T; Cane DE; Khosla C A Turnstile Mechanism for the Controlled Growth of Biosynthetic Intermediates on Assembly Line Polyketide Synthases . ACS Cent. Sci 2016, 2 ( 1 ), 14–20. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(4) Keatinge-Clay AT The Structures of Type I Polyketide Synthases . Nat. Prod. Rep 2012, 29 ( 10 ), 1050–1073. [ PubMed ] [ Google Scholar ]
(5) Tsai SC; Miercke LJ; Krucinski J; Gokhale R; Chen JC; Foster PG; Cane DE; Khosla C; Stroud RM Crystal Structure of the Macrocycle-Forming Thioesterase Domain of the Erythromycin Polyketide Synthase: Versatility from a Unique Substrate Channel . Proc. Natl. Acad. Sci. U. S. A 2001, 98 ( 26 ), 14808–14813. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(6) Donadio S; Staver MJ; McAlpine JB; Swanson SJ; Katz L Modular Organization of Genes Required for Complex Polyketide Biosynthesis . Science 1991, 252 ( 5006 ), 675 LP – 679. [ PubMed ] [ Google Scholar ]
(7) Cortes J; Haydock SF; Roberts GA; Bevitt DJ; Leadlay PF An Unusually Large Multifunctional Polypeptide in the Erythromycin-Producing Polyketide Synthase of Saccharopolyspora Erythraea . Nature 1990, 348 ( 6297 ), 176–178. [ PubMed ] [ Google Scholar ]
(8) Walker MC; Thuronyi BW; Charkoudian LK; Lowry B; Khosla C; Chang MCY Expanding the Fluorine Chemistry of Living Systems Using Engineered Polyketide Synthase Pathways . Science 2013, 341 ( 6150 ), 1089 LP – 1094. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(9) Dodge GJ; Maloney FP; Smith JL Protein-Protein Interactions in “Cis-AT” Polyketide Synthases . Nat. Prod. Rep 2018, 35 ( 10 ), 1082–1096. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(10) Klaus M; Grininger M Engineering Strategies for Rational Polyketide Synthase Design . Nat. Prod. Rep 2018, 35 ( 10 ), 1070–1081. [ PubMed ] [ Google Scholar ]
(11) Milligan JC; Lee DJ; Jackson DR; Schaub AJ; Beld J; Barajas JF; Hale JJ; Luo R; Burkart MD; Tsai S-C Molecular Basis for Interactions between an Acyl Carrier Protein and a Ketosynthase . Nat. Chem. Biol 2019, 15 ( 7 ), 669–671. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(12) Nguyen C; Haushalter RW; Lee DJ; Markwick PRL; Bruegger J; Caldara-Festin G; Finzel K; Jackson DR; Ishikawa F; O’Dowd B; McCammon JA; Opella SJ; Tsai S-C; Burkart MD Trapping the Dynamic Acyl Carrier Protein in Fatty Acid Biosynthesis . Nature 2014, 505 ( 7483 ), 427–431. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(13) Miyanaga A; Iwasawa S; Shinohara Y; Kudo F; Eguchi T Structure-Based Analysis of the Molecular Interactions between Acyltransferase and Acyl Carrier Protein in Vicenistatin Biosynthesis . Proc. Natl. Acad. Sci 2016, 113 ( 7 ), 1802 LP – 1807. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(14) Miyanaga A; Ouchi R; Ishikawa F; Goto E; Tanabe G; Kudo F; Eguchi T Structural Basis of Protein-Protein Interactions between a Trans-Acting Acyltransferase and Acyl Carrier Protein in Polyketide Disorazole Biosynthesis . J. Am. Chem. Soc 2018, 140 ( 25 ), 7970–7978. [ PubMed ] [ Google Scholar ]
(15) Mindrebo JT; Patel A; Kim WE; Davis TD; Chen A; Bartholow TG; La Clair JJ; McCammon JA; Noel JP; Burkart MD Gating Mechanism of Elongating Beta-Ketoacyl-ACP Synthases . Nat. Commun 2020, 11 ( 1 ), 1727. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(16) Skiba MA; Sikkema AP; Moss NA; Lowell AN; Su M; Sturgis RM; Gerwick L; Gerwick WH; Sherman DH; Smith JL Biosynthesis of T-Butyl in Apratoxin A: Functional Analysis and Architecture of a PKS Loading Module . ACS Chem. Biol 2018, 13 ( 6 ), 1640–1650. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(17) Dutta S; Whicher JR; Hansen DA; Hale WA; Chemler JA; Congdon GR; Narayan ARH; Håkansson K; Sherman DH; Smith JL; Skiniotis G Structure of a Modular Polyketide Synthase . Nature 2014, 510 , 512. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(18) Whicher JR; Dutta S; Hansen DA; Hale WA; Chemler JA; Dosey AM; Narayan ARH; Håkansson K; Sherman DH; Smith JL; Skiniotis G Structural Rearrangements of a Polyketide Synthase Module during Its Catalytic Cycle . Nature 2014, 510 , 560. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(19) Edwards AL; Matsui T; Weiss TM; Khosla C Architectures of Whole-Module and Bimodular Proteins from the 6-Deoxyerythronolide B Synthase . J. Mol. Biol 2014, 426 ( 11 ), 2229–2245. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(20) Li X; Sevillano N; La Greca F; Deis L; Liu Y-C; Deller MC; Mathews II; Matsui T; Cane DE; Craik CS; Khosla C Structure-Function Analysis of the Extended Conformation of a Polyketide Synthase Module . J. Am. Chem. Soc 2018, 140 ( 21 ), 6518–6521. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(21) Li X; Sevillano N; La Greca F; Hsu J; Mathews II; Matsui T; Craik CS; Khosla C Discovery and Characterization of a Thioesterase-Specific Monoclonal Antibody That Recognizes the 6-Deoxyerythronolide B Synthase . Biochemistry 2018, 57 ( 43 ), 6201–6208. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(22) Tang Y; Kim C-Y; Mathews II; Cane DE; Khosla C The 2.7-Å Crystal Structure of a 194-KDa Homodimeric Fragment of the 6-Deoxyerythronolide B Synthase . Proc. Natl. Acad. Sci 2006, 103 ( 30 ), 11124 LP – 11129. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(23) Tang Y; Chen AY; Kim C-Y; Cane DE; Khosla C Structural and Mechanistic Analysis of Protein Interactions in Module 3 of the 6-Deoxyerythronolide B Synthase . Chem. Biol 2007, 14 ( 8 ), 931–943. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(24) Whicher JR; Smaga SS; Hansen DA; Brown WC; Gerwick WH; Sherman DH; Smith JL Cyanobacterial Polyketide Synthase Docking Domains: A Tool for Engineering Natural Product Biosynthesis . Chem. Biol 2013, 20 ( 11 ), 1340–1351. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(25) Kim J; Stroud RM; Craik CS Rapid Identification of Recombinant Fabs That Bind to Membrane Proteins . Methods 2011, 55 ( 4 ), 303–309. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(26) Ostrowski MP; Cane DE; Khosla C Recognition of Acyl Carrier Proteins by Ketoreductases in Assembly Line Polyketide Synthases . J. Antibiot. (Tokyo) 2016, 69 ( 7 ), 507–510. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(27) Ostergaard LH; Kellenberger L; Cortes J; Roddis MP; Deacon M; Staunton J; Leadlay PF Stereochemistry of Catalysis by the Ketoreductase Activity in the First Extension Module of the Erythromycin Polyketide Synthase . Biochemistry 2002, 41 ( 8 ), 2719–2726. [ PubMed ] [ Google Scholar ]
(28) Lowry B; Robbins T; Weng C-H; O’Brien RV; Cane DE; Khosla C In Vitro Reconstitution and Analysis of the 6-Deoxyerythronolide B Synthase . J. Am. Chem. Soc 2013, 135 ( 45 ), 16809–16812. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(29) Keatinge-Clay AT; Stroud RM The Structure of a Ketoreductase Determines the Organization of the Beta-Carbon Processing Enzymes of Modular Polyketide Synthases . Structure 2006, 14 ( 4 ), 737–748. [ PubMed ] [ Google Scholar ]
(30) Agledal L; Niere M; Ziegler M The Phosphate Makes a Difference: Cellular Functions of NADP . Redox Rep . 2010, 15 ( 1 ), 2–10. [ PMC free article ] [ PubMed ] [ Google Scholar ]
(31) Kharel MK; Pahari P; Shaaban KA; Wang G; Morris C; Rohr J Elucidation of Post-PKS Tailoring Steps Involved in Landomycin Biosynthesis . Org. Biomol. Chem 2012, 10 ( 21 ), 4256–4265. [ PubMed ] [ Google Scholar ]
(32) Svergun D; Barberato C; Koch MHJ CRYSOL - a Program to Evaluate X-Ray Solution Scattering of Biological Macromolecules from Atomic Coordinates . J. Appl. Crystallogr 1995, 28 ( 6 ), 768–773. [ Google Scholar ]