摘要:
目的
采用水提醇沉法提取红花多糖（safflower polysaccharides，SPS），并分析其结构和体外抗氧化活性。
方法
红花粗多糖经脱蛋白、DEAE-52纤维素和Sephadex G-200凝胶分离纯化后，得到SPS-A、SPS-B和SPS-C 3个组分，采用紫外—可见光谱扫描和红外光谱对其进行分析，并考察SPS-A的体外抗氧化能力。
结果
SPS-A、SPS-B和SPS-C含量分别为89.75%，90.37%，90.22%，样品中不含蛋白质或核酸类物质，推测SPS-A、SPS-B、SPS-C为
β
-型吡喃环结构。SPS-A清除DPPH自由基的IC
50
值为0.35 mg/mL，Fe
3+
总抗氧化能力（FRAP）为411.58 μmol/L FeSO
4
。
结论
木瓜蛋白酶-Sevag法对红花粗多糖的脱蛋白效果最佳；红花多糖可能含
β
-型吡喃环结构；红花多糖可作为一种新型抗氧化药物及食物开发。
Abstract:
Objective
This study aimed to extract Safflower polysaccharides (SPS) via water extraction and alcohol precipitation method, and to analyze their structural characteristics and antioxidant activity
in vitro
.
Methods
SPS was separated into three fractions-SPS-A, SPS-B and SPS-C using DEAE-52 ion exchange and Sephadex G-200 gel chromatography. Structural properties were analyzed through UV-vis scanning and infrared spectroscopy. The antioxidant activity of SPS-A was assessed through DPPH radical scavenging and ferric reducing antioxidant power (FRAP) assays.
Results
The polysaccharide contents of SPS-A, SPS-B and SPS-C was 89.75%, 90.37%, 90.22%, respectively. The samples did not contain protein or nucleic acid substances. It is speculated that SPS-A, SPS-B and SPS-C were
β
-type pyran ring structures. The IC
50
value of SPS-A for scavenging DPPH free radicals was 0.35 mg/mL, and the total antioxidant capacity of Fe
3+
(FRAP) was 411.58 μmol/L FeSO
4
.
Conclusion
The papain-Sevag method was the most effective for deproteinization of crude polysaccharides. Safflower polysaccharide, characterized by
β
-type pyranose ring structures, demonstrated significant antioxidant potential, which can be enhanced by increasing polysaccharide concentration. Optimizing separation and purification processes could improve sample yield and support large-scale production. SPS holds promise as a novel antioxidant for pharmaceutical and functional food applications.
张馨心,王福玲.红花多糖纯化工艺及体外抗氧化活性研究[J].食品与机械,2025,41(3):171-176.
ZHANG Xinxin, WANG Fuling. Purification process and antioxidant activity
in vitro
of polysaccharides from safflower[J]. Food & Machinery,2025,41(3):171-176.
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