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- Anti-infection 抗感染
- ADC Related 抗体偶联药物相关
- Apoptosis 凋亡
- Autophagy 自噬
- Cell Cycle/DNA Damage 细胞周期/DNA 损伤
- Cytoskeleton 细胞骨架
- Epigenetics 表观遗传学
- GPCR/G Protein G 蛋白偶联受体/G 蛋白
- Immunology/Inflammation 免疫及炎症
- JAK/STAT Signaling JAK/STAT 信号通路
- MAPK/ERK Pathway MAPK/ERK 信号通路
- Membrane Transporter/Ion Channel 跨膜转运
- Metabolic Enzyme/Protease 代谢酶/蛋白酶
- Neuronal Signaling 神经信号通路
- NF-κB NF-κB 信号通路
- PI3K/Akt/mTOR PI3K/Akt/mTOR 信号通路
- PROTAC 蛋白降解靶向嵌合体
- Protein Tyrosine Kinase/RTK 蛋白酪氨酸激酶
- Stem Cell/Wnt 干细胞及 Wnt 通路
- TGF-beta/Smad TGF-beta/Smad 信号通路
- Vitamin D Related/Nuclear Receptor 维生素 D 相关/核受体
- Others 其他
Anti-infection
Cell Cycle/DNA Damage
GPCR/G Protein
- 5-HT Receptor
- Adenylate Cyclase
- Adrenergic Receptor
- Amylin Receptor
- Angiotensin Receptor
- Apelin Receptor (APJ)
- Arrestin
- Bombesin Receptor
- Bradykinin Receptor
- Cannabinoid Receptor
- CaSR
- CCR
- CGRP Receptor
- Cholecystokinin Receptor
- CRFR
- CXCR
- EBI2/GPR183
- Endothelin Receptor
- Formyl Peptide Receptor (FPR)
- Free Fatty Acid Receptor
Immunology/Inflammation
Membrane Transporter/Ion Channel
Metabolic Enzyme/Protease
- 11β-HSD
- 15-PGDH
- 17β-HSD
- 5 alpha Reductase
- Acetolactate Synthase (ALS)
- Acetyl-CoA Carboxylase
- Acyltransferase
- Adiponectin Receptor
- Aldehyde Dehydrogenase (ALDH)
- Aldose Reductase
- Aminoacyl-tRNA Synthetase
- Aminopeptidase
- Amylases
- Angiotensin-converting Enzyme (ACE)
- Arginase
- ATGL
- ATP Citrate Lyase
- Carbonic Anhydrase
- Carboxypeptidase
- Cathepsin
Neuronal Signaling
Protein Tyrosine Kinase/RTK
- Cytokines and Growth Factors 细胞因子和生长因子
- Immune Checkpoint Proteins 免疫检查点蛋白
- CAR-T related Proteins CAR-T 相关蛋白
- CD Antigens CD 抗原
- Fc Receptors Fc 受体蛋白
- Receptor Proteins 受体蛋白
- Enzymes & Regulators 酶和调节子
- Complement System 补体系统
- Ubiquitin Related Proteins 泛素相关蛋白
- Viral Proteins 病毒蛋白
- Biotinylated Proteins 生物素标记蛋白
- Fluorescent-labeled Proteins 荧光标记蛋白
- GMP-grade Proteins GMP 级蛋白
- Animal-free Recombinant Proteins 无动物成分重组蛋白
- Others 其他
- View More
CAR-T related Proteins
- B Cell Maturation Antigen (BCMA)
- FLK-1/VEGFR-2
- B7-H3
- CD4
- CD19
- CD123
- CD138/Syndecan-1
- Epithelial Cell Adhesion Molecule (EpCAM)
- Folate Receptor 1
- GPC-3
- Guanylate Cyclase 2C
- ErbB2/HER2
- ErbB3/HER3
- c-Met/HGFR
- MSLN
- CA-125
- ROR1
- CEACAM-5
- Natural Killer Group 2, Member D (NKG2D)
- Prostate Specific Membrane Antigen
- CRACC/SLAMF7
- TROP-2
- Siglec-6
- Folate Receptor alpha (FR-alpha)
- CD33
- CD70
- CD138/Syndecan-1
- CD138/Syndecan-1
- Nectin-4
- Carbonic Anhydrase 9 (CA IX)
- EGFR
- FLK-1/VEGFR-2
- CD7
- CD20
- Siglec-2/CD22
- CD30
- CD38
- MUC-1/CD227
CD Antigens
- T Cell CD Proteins
- B Cell CD Proteins
- NK Cell CD Proteins
- Macrophage CD Proteins
- Monocyte CD Proteins
- Stem Cell CD Proteins
- Platelet CD Proteins
- Erythrocyte CD Proteins
- Dendritic Cell CD Proteins
- Epithelial cell CD Proteins
- Endothelial cell CD Proteins
- Signal Transduction-related CD Proteins
- Cell Adhesion-related CD Proteins
Receptor Proteins
- Receptor Tyrosine Kinases
- Receptor Serine/Threonine Kinases
- Receptor Tyrosine Phosphatase
- Receptor Guanylyl Cyclase Family
- Cell Adhesion Molecules (CAMs)
- G-Protein-Coupled Receptors (GPCRs)
- Nuclear Receptor Superfamily
- Pattern Recognition Receptors
- Notch family
- Siglec
- Leukocyte Immunoglobin-like Receptors
- Killer-Cell Immunoglobulin-like Receptors
Enzymes & Regulators
- Oxidoreductases (EC 1)
- Transferases (EC 2)
- Hydrolases (EC 3)
- Lyases (EC 4)
- Isomerases (EC 5)
- Ligases (EC 6)
- Translocases (EC 7)
- Matrix Metalloproteinases
- ADAMs/ADAMTSs
- Cathepsin
- Carboxypeptidase
- Angiotensin-converting Enzymes
- Caspase
- Carbonic Anhydrase
- Serine/Threonine Kinase Proteins
- Protein Tyrosine Kinases
- Phosphatase
- Topoisomerase
- Protease Inhibitors
- Protein Kinase Inhibitor Peptide (PKI)
- Cyclin-Dependent Kinase Inhibitor Proteins
- Cystatin Family
Matrigel
ADMEM/F12, 青霉素/链霉素, L-谷氨酸, B27, N2, BSA, EGF, Noggin, R-spondin-1, 胃泌素, FGF10, FGF-basic, Wnt3A, 前列腺素 E2, 烟酰胺
A-83-01
Y27632
SB202190 经典人肝类器官分离培养基 : ADMEM/F12、青霉素/链霉素、谷氨酰胺、HEPES、B27(不含维A)、N2、N-乙酰半胱氨酸、烟酰胺、胃泌素1、EGF、FGF10、HGF、forskolin、R-spondin-1、Wnt3A、Noggin
Tumoroid-specific 分离培养基 :
经典的人肝类器官分离培养基，去除 R-spondin-1, Wnt3A 和 Noggin，添加地塞米松 A-83-01
Y27632 Matrigel ADMEM/F12，青霉素/链霉素，谷氨酰胺，HEPES, B27，N-乙酰半胱氨酸，EGF, R-spondin-1，胃泌素1, Wnt3A, Noggin 和 FGF A-83-01 (reduced growth factor) ADMEM/F12，青霉素/链霉素，谷氨酰胺，HEPES, B27，n-乙酰半胱氨酸，R-spondin-1, FGF7, FGF10，烟酰胺，Noggin, Primoin和 Neuregulin 1 A-83-01
Y27632 Matrige 含有 EGF, FBS, GlutaMAX 和 Primoin 的肝细胞培养基 Y27632 Matrigel
(growth factor reduced) ADMEM，青霉素/链霉素，primocin, GlutaMAX, B27, EGF，N-乙酰半胱氨酸，FGF10，碱性FGF，烟酰胺，睾酮，前列腺素E2, Noggin 和 R-spondin A-83-01
SB202190 Human FGF-2/4/9/10
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Y27632
SB202190 经典人肝类器官分离培养基 : ADMEM/F12、青霉素/链霉素、谷氨酰胺、HEPES、B27(不含维A)、N2、N-乙酰半胱氨酸、烟酰胺、胃泌素1、EGF、FGF10、HGF、forskolin、R-spondin-1、Wnt3A、Noggin
Tumoroid-specific 分离培养基 :
经典的人肝类器官分离培养基，去除 R-spondin-1, Wnt3A 和 Noggin，添加地塞米松 A-83-01
Y27632 Matrigel ADMEM/F12，青霉素/链霉素，谷氨酰胺，HEPES, B27，N-乙酰半胱氨酸，EGF, R-spondin-1，胃泌素1, Wnt3A, Noggin 和 FGF A-83-01 (reduced growth factor) ADMEM/F12，青霉素/链霉素，谷氨酰胺，HEPES, B27，n-乙酰半胱氨酸，R-spondin-1, FGF7, FGF10，烟酰胺，Noggin, Primoin和 Neuregulin 1 A-83-01
Y27632 Matrige 含有 EGF, FBS, GlutaMAX 和 Primoin 的肝细胞培养基 Y27632 Matrigel
(growth factor reduced) ADMEM，青霉素/链霉素，primocin, GlutaMAX, B27, EGF，N-乙酰半胱氨酸，FGF10，碱性FGF，烟酰胺，睾酮，前列腺素E2, Noggin 和 R-spondin A-83-01
SB202190 Human FGF-2/4/9/10
FGFs 在早期发育中对中胚层诱导、肢体发育、神经诱导和神经发育有重要作用。在成熟组织中，FGFs 对血管生成、角质形成细胞组织和伤口愈合过程至关重要。
例如 FGF-basic (FGF-2)、FGF-10 是常见的类器官培养 Niche 因子，如肝脏类器官、前列腺类器官。
1. Hanxiao Xu,Dechao Jiao,Aiguo Liu,Kongming Wu. Tumor organoids: applications in cancer modeling and potentials in precision medicine. J Hematol Oncol. 2022; 15, 58.
2. Malia Alexandra Foo, Mingliang You, Lingzhi Wang, Boon‑Cher Goh, et al. Clinical translation of patient-derived tumor organoids- bottlenecks and strategies Preclinical tumor models are an important platform for mechanistic research and testing of new drugs.Biomark Res. 2022 Mar 10;10(1):10. 3. Jarno Drost, Hans Clevers.Organoids in cancer research.Nat Rev Cancer. 2018 Jul;18(7):407-418. 4. Bauer L. LeSavage, Sarah C. Heilshorn, et al. Next-generation cancer organoids. Nat Mater. 2022 Feb;21(2):143-159. 5. Zilong Zhou, Lele Cong, Xianling Cong. Patient-Derived Organoids in Precision Medicine: Drug Screening, Organoid-on-a-Chip and Living Organoid Biobank. Front Oncol. 2021 Dec 30;11:762184. 6. Colin Rae, Francesco Amato, Chiara Braconi. Patient-Derived Organoids as a Model for Cancer Drug Discovery.Int J Mol Sci. 2021 Mar 27;22(7):3483. 7. Georgios Vlachogiannis, Nicola Valeri, et al. Patient-derived organoids model treatment response of metastatic gastrointestinal cancers. Science. 2018 Feb 23;359(6378):920-926.
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