Source name
Total RNA purchased from ADMET Technologies (Durham, NC), extracted from healthy liver tissue.
Organism
Homo sapiens
Characteristics
disease state: normal (control)
tissue: liver
Extracted molecule
total RNA
Extraction protocol
Samples were stored in RNAlater at -80 freezer before total RNA extraction with Qiagen RNeasy kit.
Label
biotin
Label protocol
Complementary DNA was prepared from total RNA.
In vitro transcription was then performed with biotin labeled substrate to produce biotin labeled cRNA.
Hybridization protocol
1, Fragmentation of cRNA.
2, Denature cRNA at 90C for 5 min.
3, Hybridization was performed at 37 C, for 18-24 hours.
4, Detection with Cy5-streptavidin conjugate.
Scan protocol
Microarray scanning and data acquisition were done at the gene expression facility, Roswell Park Cancer Institute, following their routine standard procedure.
Data processing
Data were median-normalized for comparison. Medium normalization were done by our collaborators at Roswell Park cancer institute and verified using MS Excel2003.
