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Now does the lst in preprocessor uses the same memory of lst in jsr223 sampler or it creates its new memory and uses. Q2) Another question is, does the lst memory in preprocessor gets cleared for every iteration or does it created new memory for each iteration.

In your setup you always refer to the same object which lives in JMeterVariables class instance, it neither allocates new portion of memory nor frees it during new iterations.

However be aware that each JMeter thread (virtual user) will have this object in its local storage so for 1 thread you will have 1 instance, for 2 threads - 2 instances.

So if you have > 1 thread and use the same object across all threads - it's better to use props instead of vars, as per documentation :

Properties are not the same as variables. Variables are local to a thread; properties are common to all threads

If you want to clear the object manually use vars.remove() function where needed like:

vars.remove('lst')
        
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