Characteristics
cell type: B cell lymphoma cell line
cell line: KM-H2
genotype/variation: stably transfected by KLF4ER fusion gene
treatment group: control
Treatment protocol
KM-H2-KLF4ER and L428-KLF4ER cells were incubated with 200 µM 4-OHT or with relevant amount of the vehicle (ethanol). The experiment was done twice. After 24 h of incubation total RNA was isolated with RNeasy mini kit (QIAGEN).
Growth protocol
KM-H2 and L428 cHL cells were grown in completete RPMI1640 medium at 37 ºC, 5% CO2
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated with RNeasy mini kit (QIAGEN)
Label
biotin
Label protocol
The total RNAs were amplified and labeled following the Whole Transcript (WT) Sense Target Labeling Assay (
http://www.affymetrix.com
).
Hybridization protocol
Labeled ssDNA was hybridized to Human Gene 1.0 ST Affymetrix GeneChip arrays (Affymetrix, Santa Clara, CA).
Scan protocol
The chips were scanned with a Affymetrix GeneChip Scanner 3000 according to the Affimetrix protocol.
Description
Gene expression data from KM-H2-KLF4ER cells incubated with vehicle only for 24 hours
Data processing
The microarray data were processed using GeneSifter software (
http://www.geospiza.com
) using RMA normalization protocol.
